Specification
| Target | RFP |
| Conjugate | Fluor594(Ex: 592nm, Em: 614nm), 1 moles Fluor594 per mole VHH |
| Application | ELISA,ICC/IF,Flow Cyt |
| Conjugate | Fluor594 |
| Host | Alpaca pacous |
| Isotype | VHH domain of alpaca IgG2b/2c |
| Reactivity | RFP |
| Purificatin | Affinity Purification |
| Concentration | 1mg/ml |
| Buffer | Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol |
| Storage | For long-term storage, keep at -20°C. Avoid repeated freeze/thaw cycles. |
| Advantages | · High affinity · Lot to lot consistency · Short Incubation |
Background
| Background | VHH or Nanobody is the next generation monoclonal antibody, which is a Single domain antibody (sdAb) reserving only one single antigen recognizing domain from camelid heavy chain antibody. With size of only ~ 15 kDa, Nanobody dispay numerous advantages over conventional antibody and it's derivatives, such as higher tissue penetration capability, more accessible to hidden epitopes, higher stability(thermal and pH resistant), solubility (aggregation avoidance) and binding affinity. Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |